extracellular potassium activity Search Results


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Journal: Cell reports

Article Title: TALK-1-mediated alterations of β-cell mitochondrial function and insulin secretion impair glucose homeostasis on a diabetogenic diet

doi: 10.1016/j.celrep.2024.113673

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Article Snippet: Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:500; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:250; sc-55565, Santa Cruz Biotechnology Inc., Dallas, TX, USA), and TALK-1 (dilution 1:175; APC-170, Alomone Labs, Jerusalem, Israel; or dilution 1:175; Prestige Antibodies Novus Biologicals #NBP1–83071) followed by secondary antibodies (dilution 1:500; anti-guinea pig Alexa Fluor 647, dilution 1:500; anti-mouse Alexa Fluor 488, and dilution 1:500; anti-rabbit Alexa Fluor 546). β-cell proliferation in response to HFD feeding was quantified by immunostaining for Ki-67 (dilution 1:300; ab15580, Abcam) and utilizing CytoNuclear FL v1.2 algorithm macros generated in HALO software (Indica Labs) to count insulin and Ki-67 double-positive cells.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software